Plant Transcription Factor Database
Previous version: v3.0
Oryza nivara
HSF Family
Species TF ID Description
ONIVA01G22600.1HSF family protein
ONIVA01G26320.1HSF family protein
ONIVA01G35260.1HSF family protein
ONIVA02G10840.1HSF family protein
ONIVA02G18700.1HSF family protein
ONIVA02G20850.1HSF family protein
ONIVA03G04400.1HSF family protein
ONIVA03G05710.1HSF family protein
ONIVA03G20760.1HSF family protein
ONIVA03G20850.1HSF family protein
ONIVA03G35150.1HSF family protein
ONIVA03G35150.2HSF family protein
ONIVA03G35150.3HSF family protein
ONIVA03G38630.1HSF family protein
ONIVA03G43540.1HSF family protein
ONIVA04G20610.1HSF family protein
ONIVA05G24200.1HSF family protein
ONIVA05G24200.2HSF family protein
ONIVA06G01190.1HSF family protein
ONIVA06G01190.2HSF family protein
ONIVA06G21600.1HSF family protein
ONIVA06G22540.1HSF family protein
ONIVA07G03730.1HSF family protein
ONIVA07G23600.1HSF family protein
ONIVA08G19780.1HSF family protein
ONIVA08G25630.1HSF family protein
ONIVA09G18150.1HSF family protein
ONIVA10G09550.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645